Green Synthesis of Silver Nanoparticles Using Punica granatum Peel Extract and Their Cytotoxic Effects on Human Melanoma RPMI-7951 Cells
1 Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania
2 Research Centre for Pharmaco-Toxicological Evaluation, Faculty of Pharmacy, “Victor Babeș” University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, 300041 Timișoara, Romania
3 Doctoral School, “Victor Babeș” University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, 300041 Timișoara, Romania
4 email:
* Author to whom correspondence should be addressed.
Received: 25 Aug 2025 / Accepted: 9 Sep 2025 / Published: 11 Sep 2025
Abstract
Background: Melanoma is the most aggressive skin cancer, and the development of new therapeutic strategies is essential. The synthesis of silver nanoparticles (AgNPs) using Punica granatum (PG) peel extract offers a sustainable approach with potential biomedical applications. Methods: The human melanoma cell line RPMI-7951 was used in this study to assess the effects of AgNPs which were produced with ethanolic PG peel extract. The MTT assay was used to measure cytotoxicity, and morphological analysis, Hoechst, and mitochondrial staining were used to assess the cellular response. Results: PG-AgNPs induced a dose-dependent reduction in cell viability, with significant effects observed at concentrations of 25 and 30 μg/mL. Treated cells showed decreased confluency, shrinkage, chromatin condensation, and mitochondrial changes. Conclusions: These findings demonstrate that PG-AgNPs exert cytotoxic effects on melanoma cells and highlight their potential as promising candidates for anticancer therapies, supporting further validation in in vivo models.
Keywords: ; silver nanoparticles; green synthesis; melanoma; cytotoxicity; apoptosis; nanomedicine
OPEN ACCESS
This is an open access article distributed under the Creative Commons Attribution
License which permits unrestricted use, distribution, and reproduction in any medium,
provided the original work is properly cited. (CC BY 4.0).
CITE
Predescu, I.-A.; Semenescu, A.-D.; Pătrașcu, D. Green Synthesis of Silver Nanoparticles Using Punica granatum Peel Extract and Their Cytotoxic Effects on Human Melanoma RPMI-7951 Cells. jept 2025, 2, 1.
Predescu I-A, Semenescu A-D, Pătrașcu D. Green Synthesis of Silver Nanoparticles Using Punica granatum Peel Extract and Their Cytotoxic Effects on Human Melanoma RPMI-7951 Cells. Journal of Experimental Pharmacology and Toxicology. 2025; 2(2):1.
Predescu, Iasmina-Alexandra; Semenescu, Alexandra-Denisa; Pătrașcu, Dalia. 2025. "Green Synthesis of Silver Nanoparticles Using Punica granatum Peel Extract and Their Cytotoxic Effects on Human Melanoma RPMI-7951 Cells." jept 2, no. 2: 1.
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